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pbluescript ii sk plasmid dna  (Addgene inc)


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    Addgene inc pbluescript ii sk plasmid dna
    Pbluescript Ii Sk Plasmid Dna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pbluescript ii sk plasmid dna/product/Addgene inc
    Average 93 stars, based on 39 article reviews
    pbluescript ii sk plasmid dna - by Bioz Stars, 2026-03
    93/100 stars

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    PMB had high affinity for <t>DNA</t> and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of <t>pBlueScript</t> II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.
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    PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

    Journal: Archives of toxicology

    Article Title: Polymyxin B causes DNA damage in HK-2 cells and mice

    doi: 10.1007/s00204-018-2192-1

    Figure Lengend Snippet: PMB had high affinity for DNA and inhibited electrophoretic migration of plasmid at higher concentrations. (A) 60 ng of pBlueScript II DNA incubated with PMB (12.5–500 μM) in a topoisomerase I buffer for 2 h at 37 °C. Samples were resolved by electrophoresis. (B) Chemical structure of PMB with potential DNA binding sites (including the Dab residues) highlighted.

    Article Snippet: In brief, a 20-μL reaction containing 60 ng pBlueScript II SK(+) plasmid DNA (Stratagene), polymyxin B (12.5–500 μM), or ddH 2 O (control) was incubated at 37 °C for 2 h in topoisomerase I buffer (NEB).

    Techniques: Migration, Plasmid Preparation, Incubation, Electrophoresis, Binding Assay